Spatial heterogeneity of dynamics of H1 linker histone

Linker histone H1 participates in maintaining higher order chromatin structures. It is a dynamic protein that binds to DNA and exchanges rapidly with a mobile pool. Therefore, the dynamics of H1 were probed in the nuclei of intact, live cells, using an array of microscopy techniques: fluorescence recovery after photobleaching (FRAP), raster image correlation spectroscopy (RICS), fluorescence correlation spectroscopy (FCS), pair correlation functions (pCF) and fluorescence anisotropy. Combination of these techniques yielded information on H1 dynamics at small (1–100 μs: FCS, RICS, anisotropy), moderate (1–100 ms: FCS, RICS, pCF) and large (1–100 s: pCF and FRAP) time scales. These results indicate that the global movement of H1 in nuclei (at distances >1 µm) occurs at the time scale of seconds and is determined by processes other than diffusion. Moreover, a fraction of H1, which remains immobile at the time scale of tenths of seconds, is detectable. However, local (at distances <0.7 µm) H1 dynamics comprises a process occurring at a short (~3 ms) time scale and multiple processes occurring at longer (10–2,500 ms) scales. The former (fast) process (corresponding probably to H1 diffusion) is more pronounced in the nuclear regions characterized by low H1 concentration, but the latter (slow, attributable to H1 binding) in the regions of high H1 concentration. Furthermore, some regions in nuclei (possibly containing dense chromatin) may constitute barriers that impair or block movement of H1 histones within short (<1 µm) distances

Experimental Indications for the Response of the Spectators to the Participant Blast

Precise momentum distributions of identified projectile fragments, formed in the reactions 238U + Pb and 238U + Ti at 1 A GeV, are measured with a high-resolution magnetic spectrometer. With increasing mass loss, the velocities first decrease as expected from previously established systematics, then level off, and finally increase again. Light fragments are on the average even faster than the projectiles. This finding is interpreted as the response of the spectators to the participant blast. The re-acceleration of projectile spectators is sensitive to the nuclear mean field and provides a new tool for investigating the equation of state of nuclear matter.Comment: 7 pages, 3 figures, background information on http://www-wnt.gsi.de/kschmidt

Water Status Evaluation on Tertiary Block for Developing Land Use Pattern and Water Management Strategies in Acid Sulfat Soil of Saleh Tidal Lowland Reclamation Areas of South Sumatera

The aimed of the research is to evaluate the water status in the tertiary block of tidal lowland for developing cropping pattern and water management strategies for food crop agriculture. The research was conducted in tidal lowland reclamation areas of Delta Saleh South Sumatera. The methodology used this research was survey and monitoring. Data analysis was done by comparing the water status availability with the potential cropping pattern. Computer model of DRAINMOD had been used to estimate the water table status and to design water table control operation at tertiary blocks. Model adaptation for dry land condition (C-typhology) showed that the best scenario was land utilization pattern of rice-corn. This paper presented monthly water management operational plan for rice crop in first cropping season (MT1) during November-February period and for corn crop in second cropping season (MT2) during May-August period

Probing the interface magnetism in the FeMn/NiFe exchange bias system using magnetic second harmonic generation

Second harmonic generation magneto-optic Kerr effect (SHMOKE) experiments, sensitive to buried interfaces, were performed on a polycrystalline NiFe/FeMn bilayer in which areas with different exchange bias fields were prepared using 5 KeV He ion irradiation. Both reversible and irreversible uncompensated spins are found in the antiferromagnetic layer close to the interface with the ferromagnetic layer. The SHMOKE hysteresis loop shows the same exchange bias field as obtained from standard magnetometry. We demonstrate that the exchange bias effect is controlled by pinned uncompensated spins in the antiferromagnetic layer.Comment: submitted to Phys. Rev. Let

Isotopic and velocity distributions of Bi produced in charge-pickup reactions of 208Pb at 1 A GeV

Isotopically resolved cross sections and velocity distributions have been measured in charge-pickup reactions of 1 A GeV 208Pb with proton, deuterium and titanium target. The total and partial charge-pickup cross sections in the reactions 208Pb + 1H and 208Pb + 2H are measured to be the same in the limits of the error bars. A weak increase in the total charge-pickup cross section is seen in the reaction of 208Pb with the titanium target. The measured velocity distributions show different contributions - quasi-elastic scattering and Delta-resonance excitation - to the charge-pickup production. Data on total and partial charge-pickup cross sections from these three reactions are compared with other existing data and also with model calculations based on the coupling of different intra-nuclear cascade codes and an evaporation code.Comment: 20 pages, 12 figures, background information on http://www-w2k.gsi.de/kschmidt

Fission-Residues Produced in the Spallation Reaction 238U+p at 1 A GeV

Fission fragments from 1 A GeV 238U projectiles irradiating a hydrogen target were investigated by using the fragment separator FRS for magnetic selection of reaction products including ray-tracing and DE-ToF techniques. The momentum spectra of 733 identified fragments were analysed to provide isotopic production cross sections, fission-fragment velocities and recoil momenta of the fissioning parent nuclei. Besides their general relevance, these quantities are also demanded for applications. Calculations and simulations with codes commonly used and recently developed or improved are compared to the data.Comment: 60 pages, 21 figures, 4 tables, 2 appendices (15 pages

A new analysis method to determine beta-decay half-lives in experiments with complex background

This paper reports the first application of a new technique to measure the beta-decay half -lives of exotic nuclei in complex background conditions. Since standard tools were not adapted to extract the relevant information, a new analysis method was developed. The time distribution of background events is established by recording time correlations in backward time. The beta half lives of the nuclides and the detection efficiency of the set-up are determined simultaneously from a least-squares fit of the ratio of the time-correlation spectra recorded in forward and in backward time, using numerical functions. The necessary numerical functions are calculated in a Monte-Carlo code using the known operation parameters of the experiment and different values for the two free parameters, half-life and detection efficiency, as input parameters.Comment: 21 pages, 9 figure

Evaporation residues produced in spallation of 208Pb by protons at 500A MeV

The production cross sections of fragmentation-evaporation residues in the reaction Pb+p at 500A MeV have been measured using the inverse-kinematics method and the FRS spectrometer (GSI). Fragments were identified in nuclear charge using ionisation chambers. The mass identification was performed event-by-event using the B-rho - TOF - Delta-E technique. Although partially-unresolved ionic charge states induced an ambiguity on the mass of some heavy fragments, production rates could be obtained with a high accuracy by systematically accounting for the polluting ionic charge states. The contribution of multiple reactions in the target was subtracted using a new, partly self-consistent code. The isobaric distributions are found to have a shape very close to the one observed in experiments at higher energy. Kinematic properties of the fragments were also measured. The total and the isotopic cross sections, including charge-pickup cross sections, are in good agreement with previous measurements. The data are discussed in the light of previous spallation measurements, especially on lead at 1 GeV

Discovery and Cross-Section Measurement of Neutron-Rich Isotopes in the Element Range from Neodymium to Platinum at the FRS

With a new detector setup and the high-resolution performance of the fragment separator FRS at GSI we discovered 57 new isotopes in the atomic number range of 60$\leq Z \leq 78$: \nuc{159-161}{Nb}, \nuc{160-163}{Pm}, \nuc{163-166}Sm, \nuc{167-168}{Eu}, \nuc{167-171}{Gd}, \nuc{169-171}{Tb}, \nuc{171-174}{Dy}, \nuc{173-176}{Ho}, \nuc{176-178}{Er}, \nuc{178-181}{Tm}, \nuc{183-185}{Yb}, \nuc{187-188}{Lu}, \nuc{191}{Hf}, \nuc{193-194}{Ta}, \nuc{196-197}{W}, \nuc{199-200}{Re}, \nuc{201-203}{Os}, \nuc{204-205}{Ir} and \nuc{206-209}{Pt}. The new isotopes have been unambiguously identified in reactions with a $^{238}$U beam impinging on a Be target at 1 GeV/u. The isotopic production cross-section for the new isotopes have been measured and compared with predictions of different model calculations. In general, the ABRABLA and COFRA models agree better than a factor of two with the new data, whereas the semiempirical EPAX model deviates much more. Projectile fragmentation is the dominant reaction creating the new isotopes, whereas fission contributes significantly only up to about the element holmium.Comment: 9 pages, 4 figure